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RNase A, Recombinant

CAT:
26-M1517-50
Size:
50 mg
Price:
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  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
RNase A, Recombinant - image 1

RNase A, Recombinant

  • Description: RNase A is an endoribonuclease that specifically cleaves the single strand RNA at the phosphodiester bond between the 5’-ribose of a nucleotide and the 3’ OH phosphate group of an adjacent pyrimidine nucleotide. The highest activity is demonstrated with single stranded RNA. The recombinant enzyme is identical to the native RNase A in amino acid sequence, structure and specifications. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA. Precipitation may occur at high concentrations (>10 mg/ml) of the enzyme, The enzyme is inhibited by diethyl pyrocarbonate (DEPC), guanidinium salts, β-mercaptoethanol, heavy metals and RNase-inhibitors. The RNase A has a high affinity to glass surfaces.
  • CAS Number: 9000-83-3
  • Shipping Conditions: Gel Pack
  • Storage Conditions: -20ºC
  • Shelf Life: 12 months