Anti-PSAP Antibody

Anti-PSAP Antibody

• Description: Mouse Monoclonal Antibody specific to PSAP
• Product Name Alternative: Proactivator polypeptide [Cleaved into: Saposin-A
• CAS Number: 9007-83-4
• Gene Name: PSAP
• NCBI Gene ID: PSAP
• UniProt: P07602
• Cellular Locus: Lysosome
• Host: Mouse
• Reactivity: Human
• Immunogen: Recombinant human PSAP.
• Target Antigen: Prosaposin
• Target: PSAP
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: IHC
• Field of Research: Cancer research
• Purification Method: Purified by immunoaffinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Tris
• Function: Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.; Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.; Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).; [Prosaposin]: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling. {UniProtKB:Q61207, PubMed:10383054}.; Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
• Additionnal Information:
  
  Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
• Storage Conditions: Store at 2-8°C. Do not freeze.
• Specificity: Human PSAP. Reactivity with other species has not been investigated.
• Formulation: Tris buffer, pH 7.3-7.7, 1% BSA,
• Buffer pH: pH 7.3-7.7
• Target Background: Prostatic Specific Acid Phosphatase (PSAP) is an enzyme found in the glandular epithelium of the prostate. PSAP levels are elevated in hyperplastic prostate and prostate carcinoma with the highest levels being detected in metastasized prostate cancer. Moderate overexpression of PSAP is also characteristic of diseases of the bone (such as Paget's disease or hyperparathyroidism), diseases of blood cells (such as sickle-cell disease), multiple myeloma, or lysosomal storage diseases such as Gaucher's disease.