Anti-HLA-F PE

Anti-HLA-F PE

• Background: HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.
• Specifications: The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Inclusion body-derived HLA-F heavy chain
• Target Antigen: HLA-F
• Isotype: IgG1
• Clone: 3D11
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL extracellular and intracellular staining.
• Validated Applications: FC
• Concentration: 0.1 mg/mL
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Lee N, Geraghty DE: HLA-F surface expression on B cell and monocyte cell lines is partially independent from tapasin and completely independent from TAP. J Immunol. 2003 Nov 15171(10):5264-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=hla-f+surface+expression+on+b+cell+and+monocyte+cell+lines+is+partially+independent+from+tapasin ,*Lee N, Ishitani A, Geraghty DE: HLA-F is a surface marker on activated lymphocytes. Eur J Immunol. 2010 Aug40(8):2308-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/20865824 ,*Goodridge JP, Burian A, Lee N, Geraghty DE: HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors. J Immunol. 2013 Oct 1191(7):3553-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/24018270 ,*Burian A, Wang KL, Finton KA, Lee N, Ishitani A, Strong RK, Geraghty DE: HLA-F and MHC-I open conformers bind natural killer cell Ig-like receptor KIR3DS1. PLoS One. 2016 Sep 2011(9):e0163297., URL: https://pubmed.ncbi.nlm.nih.gov/27649529/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.