Anti-Hu CD80 PE

Anti-Hu CD80 PE

• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Specifications: The antibody MEM-233 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Extracellular domain of human CD80 fused to human IgG1(Fc)
• Target Antigen: CD80
• Isotype: IgG1
• Clone: MEM-233
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15109(6):2545-52., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC18521,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15179(12):8128-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056355,*Campioni D, Moretti S, Ferrari L, Punturieri M, Castoldi GL, Lanza F: Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment. Haematologica. 2006 Mar91(3):364-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16531259?dopt=,*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 512:2., URL: http://www.ncbi.nlm.nih.gov/pubmed/21208424 ,*Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination. Mol Ther. 2005 May11(5):790-800., URL: http://www.ncbi.nlm.nih.gov/pubmed/15851017 ,*Derdak SV, Kueng HJ, Leb VM, Neunkirchner A, Schmetterer KG, Bielek E, Majdic O, Knapp W, Seed B, Pickl WF: Direct stimulation of T lymphocytes by immunosomes: virus-like particles decorated with T cell receptor/CD3 ligands plus costimulatory molecules. Proc Natl Acad Sci U S A. 2006 Aug 29103(35):13144-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16924110 ,*Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ: Rapamycin Conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype. J. Biomed. Biotechnol., vol. 2012, article ID 172420, 11 pages, doi:10.1155/2012/172420, URL: ,*Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 2003 Sep44(9):3906-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/12939308,*Allen JS, Pang K, Skowera A, Ellis R, Rackham C, Lozanoska-Ochser B, Tree T, Leslie RD, Tremble JM, Dayan CM, Peakman M: Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture. Diabetes. 2009 Jan58(1):138-45., URL: https://pubmed.ncbi.nlm.nih.gov/18835928/,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15179(12):8128-36., URL: https://pubmed.ncbi.nlm.nih.gov/18056355/,
• Other References: *Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar21(3):137-49., URL: https://www.ncbi.nlm.nih.gov/pubmed/12015893?ordin,*Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N: B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol. 2004 Sep 15173(6):3631-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15356107?ordin,*Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar21(3):137-49., URL: https://www.ncbi.nlm.nih.gov/pubmed/12015893?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.