Anti-Hu CD25 Biotin

Anti-Hu CD25 Biotin

• Background: CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
• Specifications: The antibody MEM-140 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells) it is lost on resting B and T lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: PHA-activated peripheral blood leucocytes
• Target Antigen: CD25
• Isotype: IgM
• Clone: MEM-140
• Applications: Flow cytometry: Recommended dilution: 1 μg/mL.
• Validated Applications: IP, FC
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Other References: *Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct28(10):1319-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/15371947?ordin,*Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov59 Suppl 1:i109-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/11053100?ordin,*Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75). Res Immunol. 1992 Jan143(1):33-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/1565844?ordina,*Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul23(3):224-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1838146?ordina,
• Storage Conditions: Store at 2-8°C. Do not freeze.