Anti-Ms CD45R APC

Anti-Ms CD45R APC

• Background: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
• Specifications: The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Cat, Mouse, Human
• Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
• Target Antigen: CD45R
• Isotype: IgG2a κ
• Clone: RA3-6B2
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: APC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May39(5):419-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/7514808,*Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May48(5):653-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/10769049,*Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul9(1):135-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/9697843,*Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr69(4):249-59., URL: https://www.ncbi.nlm.nih.gov/pubmed/16538653,*Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar7(3):293-301., URL: https://www.ncbi.nlm.nih.gov/pubmed/16429139,*Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1172(7):4298-307., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=cappione+aj+anolik+jh ,*Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. J Immunol. 2012 Sep 1189(5):2488-501, URL: https://pubmed.ncbi.nlm.nih.gov/22851707/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.