Anti-Hu CD85j Purified Low Endotoxin

Anti-Hu CD85j Purified Low Endotoxin

• Background: CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.
• Specifications: The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Hairy cell leukaemia cells
• Target Antigen: CD85j
• Isotype: IgG2b κ
• Clone: GHI/75
• Applications: Functional application: Blocking of HLA-G induced TGF-beta1 production. Flow cytometry: Recommended dilution: 1-4 µg/mL
• Validated Applications: FC, IP, WB, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Low Endotoxin
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Pulford K, Micklem K, Thomas J, Jones M, Mason DY: A 72-kD B cell-associated surface glycoprotein expressed at high levels in hairy cell leukaemia and plasma cell neoplasms. Clin Exp Immunol. 1991 Sep85(3):429-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=clin+exp+immunol+85(3)+pulford ,*Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb13(2):193-201., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=riteau+b+menier+c+dausset+j+martinozzi+s ,*Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep13(9):822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c ,*Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun65(6):841-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=banham+ah+colonna+m,*McIntire RH, Morales PJ, Petroff MG, Colonna M, Hunt JS: Recombinant HLA-G5 and -G6 drive U937 myelomonocytic cell production of TGF-beta1. J Leukoc Biol. 2004 Dec76(6):1220-8., URL: https://pubmed.ncbi.nlm.nih.gov/15459235/,
• Storage Conditions: Store at 2-8°C. Do not freeze.