Anti-PRKAR2A Purified

Anti-PRKAR2A Purified

• Background: PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
• Specifications: The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Target Antigen: PRKAR2A
• Isotype: IgM
• Clone: Hs-36
• Applications: Immunocytochemistry: Recommended dilution: 10 μg/mL membrane permeabilization (acetone) is essential.
• Validated Applications: ICC, WB
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• References & Citations: *Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan53(1):42-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15667524?dopt=,
• Other References: *Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May47(5):311-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12148547?dopt=,
• Storage Conditions: Store at 2-8°C. Do not freeze.