Anti-Bov Ig Light Chains Purified

Anti-Bov Ig Light Chains Purified

• Specifications: The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Sheep, Cow
• Immunogen: Fraction of Bovine immunoglobulin.
• Target Antigen: Ig Light Chains
• Isotype: IgG1
• Clone: IVA285-1
• Applications: Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in LaemmLi reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/mL
• Validated Applications: WB, FC, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct25(5):309-12. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17044788?dopt=,
• Storage Conditions: Store at 2-8°C. Do not freeze.