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Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified)

CAT:
716-HLS-H82E6-200ug
Size:
200 µg
Price:
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  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified) - image 1
Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified) - image 2
Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified) - image 3
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Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified)

  • Background: The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRAS (VVVGAGGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGAGGVGK peptide of the KRAS.
  • Description: Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (HLS-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAGGVGK).
  • Specifications: Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 39-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Expression Region: Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide
  • Host: HEK 293
  • Target: HLA-A*1101 & B2M & KRAS (VVVGAGGVGK)
  • Conjugation: Biotin-labeled
  • Tag: C-10×His & C-Avi
  • Source: Human
  • Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Endotoxin: 1.0 EU per μg
  • Purity: 90%
  • Bioactivity: Immobilized Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
  • Format: Powder
  • Buffer: PBS, pH7.4
  • Additives: Trehalose
  • Molecular Weight: 36.0 kDa and 11.7 kDa
  • Shipping Conditions: Room Temperature
  • Storage Conditions: -20°C

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