Anti-Ubiquitin Antibody

Anti-Ubiquitin Antibody

• Description: Rabbit Polyclonal Antibody specific to Ubiquitin
• Product Name Alternative: Anti-Ubiquitin Rabbit Polyclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: UBC
• Gene ID: 7316
• NCBI Gene ID: UBC
• Accession Number: NP_066289.3
• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Hamster, Sheep, Chicken, Monkey, Bovine, Canine, Porcine, Fish, Yeast, E. Coli, Drosophila, Xenopus
• Immunogen: Native bovine ubiquitin conjugated to KLH.
• Target Antigen: Polyubiquitin-C [Cleaved into: Ubiquitin]
• Target: Ubiquitin
• Clonality: Polyclonal
• Type: Antibody
• Applications: WB, IP, IHC
• Field of Research: Apoptosis
• Purification Method: Purified by protein A affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Additionnal Information: Immunoblotting: Use at 1-5ug/mL. A band of ~10kDa is detected.
  
  Immunohistochemistry: use at 1-10ug/mL.
  
  These are recommended concentrations.
  
  Endusers should determine optimal concentrations for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: Ubiquitin
• Formulation: PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
• Buffer pH: pH 7.2
• Target Background: Ubiquitin is a highly-conserved, 76-amino acid polypeptide expressed in all eukaryotic cells. Ubiquitination of proteins facilitates targeting of cytosolic and nuclear proteins for degradation by proteasomes. This essential cellular process involves a multi-enzyme cascade of ubiquitin- activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs), and ubiquitin-protein ligases (E3s). Ubiquitination is also involved in internalization and degradation of plasma membrane proteins such as T cell receptor subunits while still associated with membranes of the endoplasmic reticulum.