Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody

Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody

• Background: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser-774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).
• Description: Boster Bio Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (Catalog # P02536). Tested in WB, ICC applications. This antibody reacts with Mouse, Rat.
• Synonyms: B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody
• CAS Number: 9007-83-4
• Gene Name: DNM1
• UniProt: P21575
• Host: Sheep
• Reactivity: Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
• Clonality: Polyclonal
• Applications: WB, ICC
• Purification: Prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Molecular Weight: 97295 MW
• Components: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Storage Conditions: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
• Fragment: IgG
• Applications Notes: Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by λ-phosphatase treatment.
• Phospho Site: Phosphorylated, Ser774