Anti-Phospho-G3BP-1 (Ser232) Antibody

Anti-Phospho-G3BP-1 (Ser232) Antibody

• Background: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  
  Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
  Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
  Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
  Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.
• Description: Boster Bio Anti-Phospho-G3BP-1 (Ser232) Antibody (Catalog # P02199). Tested in WB, IHC, IF applications. This antibody reacts with Human.
• Synonyms: Ras GTPase-activating protein-binding protein 1;G3BP-1;3.6.4.12;3.6.4.13;ATP-dependent DNA helicase VIII;hDH VIII;GAP SH3 domain-binding protein 1;G3BP1;G3BP;
• CAS Number: 9007-83-4
• Gene Name: G3BP1
• UniProt: Q13283
• Host: Rabbit
• Reactivity: Human
• Immunogen: Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.
• Clonality: Polyclonal
• Applications: WB, IHC, IF
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Concentration: 1 mg/ml
• Form: Liquid
• Molecular Weight: 52164 MW
• Components: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.