Anti-Phospho-Parkin (Ser378) PARK2 Antibody

Anti-Phospho-Parkin (Ser378) PARK2 Antibody

• Background: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substantia nigra (Jenner et al., 1992). Recent evidence suggests that phosphorylation of parkin at Ser-378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).
• Description: Boster Bio Anti-Phospho-Parkin (Ser378) PARK2 Antibody (Catalog # P00127-1). Tested in WB applications. This antibody reacts with Human.
• Synonyms: AR JP antibody, E3 ubiquitin ligase antibody, E3 ubiquitin protein ligase parkin antibody, E3 ubiquitin-protein ligase parkin antibody, FRA6E antibody, LPRS 2 antibody, LPRS2 antibody, PARK 2 antibody, Park2 antibody, Parkin 2 antibody, Parkinson disease (autosomal recessive juvenile) 2 antibody, Parkinson disease (autosomal recessive juvenile) 2 parkin antibody, Parkinson disease protein 2 antibody, Parkinson juvenile disease protein 2 antibody, Parkinson protein 2 E3 ubiquitin protein ligase antibody, Parkinson protein 2 E3 ubiquitin protein ligase (parkin) antibody, PDJ antibody, PRKN 2 antibody, PRKN antibody, PRKN2 antibody, PRKN2_HUMAN antibody, Ubiquitin E3 ligase PRKN antibody
• CAS Number: 9007-83-4
• Gene Name: PARK2
• UniProt: O60260
• Host: Rabbit
• Reactivity: Human
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 of human Parkin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
• Clonality: Polyclonal
• Applications: WB
• Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Molecular Weight: 51641 MW
• Components: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Storage Conditions: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
• Fragment: IgG
• Applications Notes: Specific for the ~52 kDa parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.
• Phospho Site: Phosphorylated, Ser378