Anti-Myosin MYL9 Antibody

Anti-Myosin MYL9 Antibody

• Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200, 000 daltons, and four light chains each ~16, 000 - 21, 000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.
• Description: Boster Bio Anti-Myosin MYL9 Antibody (Catalog # A06446). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
• Synonyms: Myosin, Myosin regulatory light polypeptide 12A, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3
• CAS Number: 9007-83-4
• Gene Name: MYL12A
• UniProt: J3QRS3
• Host: Rabbit
• Reactivity: Mouse, Rat
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.
• Clonality: Polyclonal
• Applications: ELISA, WB
• Purification: This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Cross-reactivity is expected with myosin light chain from human, mouse and rat sources.  Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species.  BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences.  Cross-reactivity is expected.
• Concentration: 0.98mg/mL by UV absorbance at 280 nm
• Form: Liquid (sterile filtered)
• Molecular Weight: 19827 MW
• Components: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
• Storage Conditions: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
• Fragment: IgG
• Applications Notes: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.