Anti-Apc2 ANAPC2 Antibody

Anti-Apc2 ANAPC2 Antibody

• Background: APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.
• Description: Boster Bio Anti-Apc2 ANAPC2 Antibody (Catalog # A06153). Tested in IHC, WB applications. This antibody reacts with Human.
• Synonyms: Apc2, ANAPC2, Anaphase promoting complex subunit 2, APC2, KIAA1406, Acp, Cyclosome subunit 2
• CAS Number: 9007-83-4
• Gene Name: ANAPC2
• UniProt: Q9UJX6
• Host: Rabbit
• Reactivity: Human
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH.
• Clonality: Polyclonal
• Applications: IHC, WB
• Purification: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human APC2. Cross-reactivity may also occur with APC2 from other sources.  Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
• Concentration: 85 mg/mL by Refractometry
• Form: Liquid (sterile filtered)
• Components: 0.01% (w/v) Sodium Azide
• Storage Conditions: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
• Fragment: Antiserum
• Applications Notes: This antibody reacts with human APC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC11) does occur. A 93.8 kDa band corresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 can be used as a positive control. Researchers should determine optimal titers for other applications.