Anti-CD97 antigen CD97 Antibody

Anti-CD97 antigen CD97 Antibody

• Background: This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.
• Description: Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee.
• Synonyms: CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, CD97 antigen subunit alpha, CD97 antigen subunit beta
• CAS Number: 9007-83-4
• Gene Name: CD97
• UniProt: Q9Z0M6
• Host: Rabbit
• Reactivity: Human, Chimpanzee
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
• Clonality: Polyclonal
• Applications: IF, WB
• Purification: This Protein A purified antibody is directed against the mouse CD97 protein. The product was protein A purified from monospecific antiserum.  A BLAST analysis was used to suggest cross-reactivity with CD97 proteins from mouse (100%) and rat (74%).  Only 49% homology is noted for the human homologue.  Reactivity against CD97 from other sources is not known.
• Concentration: 5.0 mg/mL by UV absorbance at 280 nm
• Form: Lyophilized
• Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
• Components: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
• Storage Conditions: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
• Fragment: IgG
• Applications Notes: This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.