Mouse CCL19/MIP-3 beta ELISA Kit PicoKine®

Mouse CCL19/MIP-3 beta ELISA Kit PicoKine®

• Background: Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
• Description: Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 19;Epstein-Barr virus-induced molecule 1 ligand chemokine;EBI1 ligand chemokine;ELC;Small-inducible cytokine A19;Ccl19;Elc, Scya19;
• CAS Number: 7732-18-5
• Gene Name: C-C motif chemokine 19
• Gene ID: 24047
• UniProt: O70460
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: G26-S108
• Tissue Specificity: Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.
• Applications: ELISA
• Standard: 10 ng/tube
• Sensitivity: <10 pg/ml
• Function: Strongly chemotactic for naive (L-selectinhi) CD4 T- cells and for CD8 T-cells and weakly attractive for resting B- cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 11911 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	30	32	28	32	30	1.65	5.5%
  Sample 2	161	166	173	167	166	4.26	2.5%
  Sample 3	384	401	402	407	398	8.67	2.1%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse CCL19
• Subcellular Location: Secreted.
• Protein Name: C-C motif chemokine 19