Rat BMP-2 ELISA Kit PicoKine®

Rat BMP-2 ELISA Kit PicoKine®

• Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
• Description: Rat BMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;
• CAS Number: 7732-18-5
• Gene Name: Bone morphogenetic protein 2
• Gene ID: 29373
• UniProt: P49001
• Reactivity: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: CHO; Immunogen sequence: Q283-R396
• Tissue Specificity: Expressed in femur, calvaria, trachea, lung and ovary.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: bone tissue, cell culture supernatants and serum.
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <2 pg/ml
• Function: Induces cartilage and bone formation.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 44383 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	96	96	106	100	99	4.09	4.1%
  Sample 2	245	258	242	225	242	11.75	4.8%
  Sample 3	806	902	918	836	865	46.09	5.3%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant rat BMP-2
• Subcellular Location: Secreted .
• Protein Name: Bone morphogenetic protein 2