Anti-Human TLR1 DyLight® 550 conjugated Antibody

Anti-Human TLR1 DyLight® 550 conjugated Antibody

• Background: Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
• Description: Boster Bio Anti-Human TLR1 DyLight® 550 conjugated Antibody catalog # A00429-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
• Synonyms: Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD281; TLR1; KIAA0012
• CAS Number: 9007-83-4
• Gene Name: toll-like receptor 1
• Gene ID: 7096
• UniProt: Q15399
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E. coli-derived human TLR1 recombinant protein (Position: F350-D404).
• Clonality: Polyclonal
• Conjugation: DyLight®550
• Applications: Flow Cytometry
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Function: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
• References & Citations: 1. Hart, B. E., Tapping, R. I. Differential trafficking of TLR1 I602S underlies host protection against pathogenic mycobacteria. J. Immun. 189: 5347-5355, 2012. 2. West, A. P., Brodsky, I. E., Rahner, C., Woo, D. K., Erdjument-Bromage, H., Tempst, P., Walsh, M. C., Choi, Y., Shadel, G. S., Ghosh, S. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476-480, 2011. 3. Xu, Y., Tao, X., Shen, B., Horng, T., Medzhitov, R., Manley, J. L., Tong, L. Structural basis for signal transduction by the Toll/interleukin-1 receptor domains. Nature 408: 111-115, 2000.
• Storage Conditions: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
• Observed Molecular Weight: 39 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells