Anti-Human PPID DyLight® 488 conjugated Antibody

Anti-Human PPID DyLight® 488 conjugated Antibody

• Background: Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. The Cyclophilin D (PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.
• Description: Boster Bio Anti-Human PPID DyLight® 488 conjugated Antibody catalog # A02424-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
• Synonyms: Peptidyl-prolyl cis-trans isomerase D; PPIase D
• CAS Number: 9007-83-4
• Gene Name: peptidylprolyl isomerase D
• Gene ID: 5481
• UniProt: Q08752
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E. coli-derived human PPID recombinant protein (Position: N306-A370).
• Clonality: Polyclonal
• Conjugation: DyLight®488
• Applications: Flow Cytometry
• Purification: Immunogen affinity purified.
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA- binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large- cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.
• References & Citations: 1. Kieffer, L. J., Thalhammer, T., Handschumacher, R. E.Isolation and characterization of a 40-kDa cyclophilin-related protein.J. Biol. Chem. 267: 5503-5507, 1992. 2. Ratajczak, T., Woollatt, E., Kumar, P., Ward, B. K., Minchin, R. F., Baker, E.Cyclophilin 40 (PPID) gene map position 4q31.3.Chromosome Res. 5: 151 only, 1997. 3. Yokoi, H., Shimizu, Y., Anazawa, H., Lefebvre, C. A., Korneluk, R. G., Ikeda, J.-E.The structure and complete nucleotide sequence of the human cyclophilin 40 (PPID) gene.Genomics 35: 448-455, 1996.
• Storage Conditions: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
• Calculated Molecular Weight: 15126 MW
• Observed Molecular Weight: 39 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells