Anti-GFAP Antibody (Monoclonal, G-A-5)

Anti-GFAP Antibody (Monoclonal, G-A-5)

• Background: Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
• Description: Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
• Synonyms: Glial fibrillary acidic protein;GFAP;Gfap;
• CAS Number: 9007-83-4
• Gene Name: Glial fibrillary acidic protein
• Gene ID: Human(2670), Mouse(14580), Rat(24387)
• UniProt: P47819
• Host: Mouse
• Reactivity: Human,Mouse,Pig,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: GFAP from pig spinal cord.
• Clonality: Monoclonal
• Clone: Clone: G-A-5
• Tissue Specificity: Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .
• Applications: IF,IHC,IHC-F,WB
• Purification: Ascites
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Function: GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
• Components: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• References & Citations: 1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 49957 MW
• Observed Molecular Weight: 50 kDa
• Fragment: Mouse IgG1
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.5-1μg/ml, Human, mouse, pig, rat
  Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat, By Heat
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, -
  Immunofluorescence, 2μg/ml, Rat
  
• Subcellular Location: Cytoplasm . Associated with intermediate filaments. .
• Sequence Similarities: Belongs to the intermediate filament family.
• Protein Name: Glial fibrillary acidic protein