Anti-c-Myc Antibody (Monoclonal, 9E10)

Anti-c-Myc Antibody (Monoclonal, 9E10)

• Background: C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
• Description: Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
• Synonyms: Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc;
• CAS Number: 9007-83-4
• Gene Name: Myc proto-oncogene protein
• Gene ID: Human(4609), Mouse(17869), Rat(24577)
• UniProt: P09416
• Host: Mouse
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.
• Clonality: Monoclonal
• Clone: Clone: Clone: 9E10
• Tissue Specificity: Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
• Applications: IHC,ICC,WB
• Purification: Ascites
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Function: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .
• Components: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• References & Citations: 1. Liao D, Thakur A, Wu J, Biliran H, Sarkar FH. Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy.Crit Rev Oncog. 2007;13(2):93-158. 2. Britton S, Salles B, Calsou P. c-MYC protein is degraded in response to UV irradiation.Cell Cycle. 2007 Oct 2;7(1) 3. Chow JM, Liu CR, Lin CP, Lee CN, Cheng YC, Lin S, Liu HE. Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.Exp Hematol. 2008 Feb;36(2):140-8. 4. Huang CY, Bredemeyer AL, Walker LM, Bassing CH, Sleckman BP. Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse.Eur J Immunol. 2008 Jan 14.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 48898 MW
• Observed Molecular Weight: 49 kDa
• Fragment: Mouse IgG1
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Paraffin-embedded Section), 5μg/ml, Human, By Heat
  Immunocytochemistry , 1μg/ml, Human, -
  Western blot, 1μg/ml, Human
  
• Subcellular Location: Nucleus, nucleoplasm . Nucleus, nucleolus .
• Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.
• Protein Name: Myc proto-oncogene protein