Anti-CD8 Cd8A Antibody (Monoclonal, CA-8)

Anti-CD8 Cd8A Antibody (Monoclonal, CA-8)

• Background: The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.
• Description: Boster Bio Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) catalog # MA1017. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
• Synonyms: T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;
• CAS Number: 9007-83-4
• Gene Name: CD8A; CD8B
• Gene ID: Human(925), Mouse(12525), Rat(24930)
• UniProt: P01732
• Host: Mouse
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: Human thymocytes followed by peripheral blood T cells.
• Clonality: Monoclonal
• Clone: Clone: CA-8
• Tissue Specificity: Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
• Applications: Flow Cytometry,IHC,ICC
• Purification: Ascites
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
• Components: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• References & Citations: 1. Giblin, P.; Ledbetter, J. A.; Kavathas, P. : A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing. Proc. Nat. Acad. Sci. 86: 998-1002, 1989.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 23400 MW
• Observed Molecular Weight: 25 kDa
• Fragment: Mouse IgG2a
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Frozen Section), 1μg/ml, Human
  Immunocytochemistry, 0.5-1μg/ml, Human
  Flow Cytometry, 1-3μg/1x10⁶ cells, Human
• Subcellular Location: Membrane; Single-pass type I membrane protein.
• Sequence Similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.