MGMT Antibody

MGMT Antibody

• Description: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.
• CAS Number: 9007-83-4
• UniProt: P16455
• Host: Rabbit
• Immunogen: A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.
• Clonality: Monoclonal
• Isotype: IgG κ
• Applications: IHC-P
• Format: Purified
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Reconstitution: Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This MGMT antibody is available for research use only.