V5 tag, AlpHcAbs® Rabbit

V5 tag, AlpHcAbs® Rabbit

• Description: Anti-V5 tag, AlpHcAbs® Rabbit antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Rabbit antibody reacts with the V5 tag sequence (GKPIPNPLLGLDST) selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production.
• Host: Alpaca
• Species Reactivity: Universal
• Immunogen: V5 tag fusion protein
• Conjugation: Non-conjugated
• Type: Alpaca monoclonal Antibody
• Applications: WB, ICC/IF, ELISA, IP, Flow Cyt
• Concentration: 1 mg/mL
• Dilution: WB: 1:10,000-1:50,000 ;ELISA: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;IP: 1-2ug/sample ;Flow Cyt:1µg for 106 cells ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
• Purity: Recombinant Expression and Affinity purified
• Form: Liquid
• Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
• Storage Conditions: Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C