Human CD30 / TNFRSF8 Protein, His Tag

Human CD30 / TNFRSF8 Protein, His Tag

• Background: Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.
• Description: Human CD30, His Tag (CD0-H52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.4 kDa. The protein migrates as 53-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Phe 19 - Lys 379
• Host: HEK 293
• Target: CD30
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 0.1 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Human CD30, His Tag (Cat. No. CD0-H52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD30 Human Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 40.4 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C