DAG (Diacylglycerol) Assay Kit
CAT:
197-MET-5028
Size:
100 Assays
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: Yes
DAG (Diacylglycerol) Assay Kit
- Description: Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
- UNSPSC: 41116100
- Detection Method: Fluorometric
- Features & Benefits: Suitable for use with cell lysates; Detection sensitivity of 4uM DAG; DAG standard included
- Shipping Conditions: Dry Ice
- Storage Conditions: Store the entire kit at -80°C. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.