KCC2 Antibody: RPE

KCC2 Antibody: RPE

• Background: KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
• Description: Mouse Anti-Rat KCC2 Monoclonal IgG2a
• Specifications: Detects ~140kDa.
• Product Name Alternative: Potassium Chloride Cotransporter Antibody, Potassium chloride transporter 5 Antibody, SLC12A5 Antibody, hKCC2 Antibody, S12A5 Antibody, Solute carrier family 12 member 5 Antibody, Electroneutral potassium-chloride cotransporter 2 Antibody, Furosemide-sensitive K-Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, rKCC2 Antibody, Neuronal K-Cl cotransporter Antibody, KIAA1176 Antibody, Electroneutral potassium chloride cotransporter 2 Antibody, Erythroid K Cl cotransporter 2 Antibody, Furosemide sensitive K Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, KCC 2 Antibody, Neuronal K Cl cotransporter Antibody, Solute carrier family 12 (potassium chloride transporter) member 5 Antibody, Solute carrier family 12 member 5 Antibody
• Synonyms: KCC2 Antibody, Clone N1/12: RPE
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 171373
• Swiss Prot: Q63633
• Accession Number: NP_599190
• Cellular Locus: Membrane
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2
• Target: KCC2
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: N1/12 (Formerly sold as S1-12)
• Conjugation: RPE
• Validated Applications: WB, IHC, ICC/IF
• Purification: Protein G Purified
• Limit Of Detection: 1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.