EF-1 α1/2 rabbit pAb

EF-1 α1/2 rabbit pAb

• Description: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008],
• Synonyms: EEF1A1; EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7; EEF1A2; EEF1AL; STN; Elongation factor 1-alpha 2; EF-1-alpha-2; Eukaryotic elongation factor 1 A-2; eEF1A-2; Statin-S1; EEF1A1P5; EEF1AL3; Putative elongation factor 1-alpha-like 3; EF-1-alpha-like 3; Eukaryotic elongation factor 1 A-like 3; eEF1A-like 3; Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5
• Gene ID: 1915/1917
• UniProt: P68104/Q05639/Q5VTE0
• Cellular Locus: Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934)..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from the N-terminal region of human EF-1 α1/2.
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
• Molecular Weight: 50kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.