GFP, Aequorea victoria (His)

GFP, Aequorea victoria (His)

• UNSPSC Description: The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli [1][2][3][4][5][6][7].
• Type: Recombinant Proteins
• Assay Protocol: https://www.medchemexpress.com/cytokines/gfp-protein-aequorea-victoria-his.html
• Purity: 98.00
• Smiles: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
• Molecular Weight: Approximately 27-31 kDa
• References & Citations: [1]Prendergast FG, et al. Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea. Biochemistry. 1978 Aug 22;17(17):3448-53.|[2]Phillips GJ. Green fluorescent protein-a bright idea for the study of bacterial protein localization. FEMS Microbiol Lett. 2001 Oct 16;204(1):9-18. |[3]Morise H, et al. Intermolecular energy transfer in the bioluminescent system of Aequorea. Biochemistry. 1974 Jun 4;13(12):2656-62. |[4]Ormö M, et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5.|[5]Song Y H, et al. Noninvasive monitoring of environmental toxicity through green fluorescent protein expressing Escherichia coli[J]. Korean Journal of Chemical Engineering, 2016, 33: 1331-1336.|[6]Terskikh A, et al. "Fluorescent timer": protein that changes color with time. Science. 2000 Nov 24;290(5496):1585-8.|[7]Kouros-Mehr H, et al. GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model. Cancer Cell. 2008 Feb;13(2):141-52.
• Shipping Conditions: Blue Ice
• Storage Conditions: Stored at -20°C for 2 years