Biotinylated Human PD-L2 / B7-DC Protein, Avitag™,His Tag (recommended for biopanning)

Biotinylated Human PD-L2 / B7-DC Protein, Avitag™,His Tag (recommended for biopanning)

• Background: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
• Description: Biotinylated Human PD-L2, Avitag,His Tag (PD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
• Specifications: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.2 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Leu 20 - Pro 219
• Host: HEK 293
• Target: PD-L2
• Conjugation: Biotin-labeled
• Tag: C-Avi & C-6×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Anti-PD-L2 MAb (human IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Avitag,His Tag (Cat. No. PD2-H82E8) with a linear range of 2-50 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 25.2 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C