Recombinant IKB alpha Monoclonal Antibody

• Background: Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor,alpha (NFKBIA,synonyms: IKBA,MAD-3,NFKBI). NFKB1 or NFKB2 is bound to REL,RELA ,or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB),which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA,or IKBKB) marks them for destruction via the ubiquitination pathway,thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
• Synonyms: I kappa B alpha,I-kappa-B-alpha,IkappaBalpha,IkB-alpha,IKBA,IKBA,IKBalpha,MAD 3,MAD3,NF kappa B inhibitor alpha,NFKBI,IKB alpha
• Gene ID: 4792
• Swiss Prot: P25963
• Host: Rabbit
• Reactivity: Human,Rat
• Immunogen: A synthetic peptide of human IKB alpha
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Applications: WB
• Purification: Affinity purification
• Concentration: 300 μg/mL
• Dilution: WB 1:1000-1:2000
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
• Storage Conditions: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
• Calculated Molecular Weight: 44kDa
• Observed Molecular Weight: 55kDa