Anti-VPS29 | Vacuolar protein sorting-associated protein 29

Anti-VPS29 | Vacuolar protein sorting-associated protein 29

• Background: VSP29 (Vacuolar protein sorting-associated protein 29)plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Cellular localisation: endosome and Golgi apparatus.Alternative names: MAIGO1, Vesicle protein sorting 29
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Recombinant, His6 tagged VPS35b ofArabidopsis thalianaUniProt:Q9STT2, TAIR:At3g47810
• Clonality: Polyclonal
• Applications: Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1: 100 (IP), 1: 1000 - 1: 3000 (WB)
• Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
• Format: Liquid at 2 mg/mL.
• Molecular Weight: 21 | 25 kDa
• References & Citations: Yamazakiet al. (2008). Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. (Immunoprecipitation, Western blot, Arabidopsis thaliana)Shimadaet al. (2006). et al. (2006). AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. Plant Cell Physiol. 2006 Sep;47(9):1187-94. doi: 10.1093/pcp/pcj103. (Western blot, Arabidopsis thaliana)
• Storage Conditions: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.