Anti-ADGP | ADP-glucose pyrophosphorylase

Anti-ADGP | ADP-glucose pyrophosphorylase

• Background: ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlowet al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kimet al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Triticum aestivum, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Part ofArabidopsis thalianarecombinant ADP-glucose pyrophosphorylase small subunit, TAIR:At5g48300, UniProt:P55228
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000-1 : 5000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 49.4 | 52 kDa
• Precautions: Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase.
• References & Citations: Limet al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Maet al. (2021) A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals. Nat Genet. 2021 Apr 29. doi: 10.1038/s41588-021-00855-6. Epub ahead of print. PMID: 33927398.Changet al. (2020). Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method. Bioresource Technology.Volume 303, May 2020, 122932.Ancinet al. (2019). NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.Plants (Basel). 2019 Nov 26;8(12). pii: E543. doi: 10.3390/plants8120543.Takahashiet al. (2019). Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses. Plant Mol Biol. 2019 Mar 25. doi: 10.1007/s11103-019-00855-5.Denget al. (2018). Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit. Front Plant Sci. 2018 Apr 10;9:425. doi: 10.3389/fpls.2018.00425. eCollection 2018.Yoshidaet al. (2018). Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8296-E8304. doi: 10.1073/pnas.1808284115.Zhenet al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.