Mouse Cytokine Chemi (4-plex) Sample Demo Kit

Mouse Cytokine Chemi (4-plex) Sample Demo Kit

• Description:

  Product Overview

  Mouse Cytokine Chemi (4-plex) Sample Kit is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 4 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 4 markers in the panel within 2.25 hours. The Mouse Cytokine Chemi (4-plex) Sample Kit provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

  *For a limited time, we are offering this kit on trial basis for only $99 per kit. Limit to 1 kit per lab. Claim yours today by contacting us at [email protected] for details.

  Assay Ranges

  	Calibrator Range	Units
  IL-2	5000-6.86	pg/ml
  IL-4	5000-6.86	pg/ml
  IL-6	5000-6.86	pg/ml
  TNFα	3000-14.12	pg/ml

  Kit Components

  Each kit contains a 96-well plate (not strip well), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

  Reagents Include:

  Calibrator: Lyophilized, recombinant antigens in a buffered protein base
  Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
  Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
  Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
  Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
  Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
  Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
  Plate Seals (3): Adhesive strips