Mouse CHIT1 ELISA Kit PicoKine®, Carrier-free

Mouse CHIT1 ELISA Kit PicoKine®, Carrier-free

• Background: Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1; 1 E4. It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract. CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form. Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease. The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme. Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
• Description: Mouse CHIT1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Chit1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Chitotriosidase-1; Chitinase-1; Chit1
• Gene Name: chitinase 1 (chitotriosidase)
• Gene ID: 71884
• UniProt: Q9D7Q1
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A22-S464
• Tissue Specificity: Highly expressed in tongue, stomach, kidney, brain, skin, testis, and bone marrow. Low level of expression was found in lung, heart, spleen, small intestine, and liver. Not detectable in pancreas, salivary gland, large intestine, uterus, or peripheral blood mononuclear cells (PBMC).
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <15 pg/ml
• Function: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse CHIT1
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	57	62	36	48	51	3.33	6.6%
  Sample 2	100	80	124	79	96	6.81	7.1%
  Sample 3	930	789	781	644	786	32.3	4.1%

  *number of samples for each test n=16.
• : heparin or EDTA
• Subcellular Location: Lysosome. Secreted.
• Protein Name: Chitotriosidase-1