Mouse CD86/B7-2 ELISA Kit PicoKine®, Carrier-free

Mouse CD86/B7-2 ELISA Kit PicoKine®, Carrier-free

• Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
• Description: Mouse CD86/B7-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd86 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: T-lymphocyte activation antigen CD86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1;CD86;Cd86;
• Gene Name: T-lymphocyte activation antigen CD86
• Gene ID: 12524
• UniProt: P42082
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: V26-G245
• Tissue Specificity: Expressed on activated B-cells.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 34666 MW
• Specificity: Natural and recombinant mouse CD86
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	179	175	168	168	172	4.71	2.7%
  Sample 2	772	787	740	767	766	16.97	2.2%
  Sample 3	1639	1511	1669	1837	1664	116.17	6.9%

  *number of samples for each test n=16.
• Subcellular Location: Cell membrane ; Single-pass type I membrane protein .
• Protein Name: T-lymphocyte activation antigen CD86