Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®, Carrier-free

Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®, Carrier-free

• Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
• Description: Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine® (96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Alpha-2-macroglobulin;Alpha-2-M;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5;A2M;CPAMD5;FWP007;
• Gene Name: Alpha-2-macroglobulin
• Gene ID: 2
• UniProt: P01023
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Target protein isolated from human plasma
• Tissue Specificity: Secreted in plasma. .
• Applications: ELISA
• Standard: 40 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 625 pg/ml - 40,000 pg/ml
• Sensitivity: <20 pg/ml
• Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural human A2M
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1367	1285	1469	1391	1378	65.61	4.7%
  Sample 2	3876	3671	3260	3321	3532	253.06	7.1%
  Sample 3	26784	32034	29630	29138	29396	1864.33	6.3%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 163291 MW
• Subcellular Location: Secreted .
• Sequence Similarities: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
• Protein Name: Alpha-2-macroglobulin