Mouse TNFRSF7/CD27 ELISA Kit PicoKine®, Carrier-free

Mouse TNFRSF7/CD27 ELISA Kit PicoKine®, Carrier-free

• Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
• Description: Mouse TNFRSF7/CD27 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: CD27 antigen;CD27L receptor;T-cell activation antigen CD27;Tumor necrosis factor receptor superfamily member 7;CD27;Cd27;Tnfrsf7;
• Gene Name: CD27 antigen
• Gene ID: 21940
• UniProt: P41272
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T21-R182
• Tissue Specificity: In thymus and spleen, but not in non-lymphoid tissues.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 28164 MW
• Specificity: Natural and recombinant mouse TNFRSF7
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	83	88	88	94	88	3.89	4.4%
  Sample 2	588	678	590	656	628	39.77	6.3%
  Sample 3	1891	1745	2088	2100	1956	147.39	7.5%

  *number of samples for each test n=16.
• Subcellular Location: Membrane; Single-pass type I membrane protein.
• Protein Name: CD27 antigen