Anti-Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal Antibody, Carrier-free

Anti-Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal Antibody, Carrier-free

• Description: Boster Bio Anti-Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal Antibody catalog # P01058-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
• Synonyms: Ribosomal protein S6 kinase alpha-1;S6K-alpha-1;2.7.11.1;90 kDa ribosomal protein S6 kinase 1;p90-RSK 1;p90RSK1;p90S6K;MAP kinase-activated protein kinase 1a;MAPK-activated protein kinase 1a;MAPKAP kinase 1a;MAPKAPK-1a;Ribosomal S6 kinase 1;RSK-1;RPS6KA1;MAPKAPK1A, RSK1;
• Gene Name: Ribosomal protein S6 kinase alpha-1
• UniProt: Q15418
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Immunogen: A synthesized peptide derived from human Phospho-RSK1 (S380)
• Clonality: Monoclonal
• Clone: Clone: EDE-18
• Tissue Specificity: Ubiquitously expressed, with more abundant expression in the brain.
• Applications: WB,ICC,IF,IP
• Purification: Affinity-chromatography
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Function: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin- derived signal, acts inly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by ly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. .
• Components: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
• Storage Conditions: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 82723 MW
• Observed Molecular Weight: 80 kDa
• Fragment: Rabbit IgG
• Applications Notes: WB 1:500-1:2000
  ICC/IF 1:50-1:200
  IP 1:50
• Phospho Site: S380
• Subcellular Location: Nucleus. Cytoplasm.