Anti-CD16/Fcgr3 Antibody Picoband®, iFluor647 Conjugate

Anti-CD16/Fcgr3 Antibody Picoband®, iFluor647 Conjugate

• Background: Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
• Description: Boster Bio Anti-CD16/Fcgr3 Antibody Picoband® catalog # A32617. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29
• Gene Name: retrotransposon Gag like 10
• Gene ID: 14131
• UniProt: P08508
• Host: Rabbit
• Reactivity: Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived mouse CD16/Fcgr3 recombinant protein (Position: A31-T215).
• Clonality: Polyclonal
• Tissue Specificity: Ubiquitously expressed.
• Applications: WB,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Could induce apoptosis in a BH3 domain-dependent manner. The interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. Pinheiro da Silva, F., Aloulou, M., Skurnik, D., Benhamou, M., Andremont, A., Velasco, I. T., Chiamolera, M., Verbeek, J. S., Launay, P., Monteiro, R. C. CD16 promotes Escherichia coli sepsis through an FcR-gamma inhibitory pathway that prevents phagocytosis and facilitates inflammation. Nature Med. 13: 1368-1374, 2007. 2. Robinson, J. I., Carr, I. M., Cooper, D. L., Rashid, L. H., Martin, S. G., Emery, P., Isaacs, J. D., Barton, A., BRAGGSS, Wilson, A. G., Barrett, J. H., Morgan, A. W. Confirmation of association of FCGR3B but not FCGR3A copy number with susceptibility to autoantibody positive rheumatoid arthritis. Hum. Mutat. 33: 741-749, 2012.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 14377 MW
• Observed Molecular Weight: 30 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor.
• Protein Name: Lymphocyte antigen 6A-2/6E-1