Anti-NDUFAF1 Antibody Picoband®, FITC Conjugate

Anti-NDUFAF1 Antibody Picoband®, FITC Conjugate

• Background: Complex I intermediate-associated protein 30, mitochondrial (CIA30) is a protein that in humans is encoded by the NDUFAF1 gene. This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.
• Description: Boster Bio Anti-NDUFAF1 Antibody Picoband® catalog # A10145. Tested in ELISA, IHC, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3;
• Gene Name: Protein NDRG3
• Gene ID: 51103
• UniProt: Q9Y375
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human NDUFAF1 recombinant protein (Position: D79-K327).
• Clonality: Polyclonal
• Tissue Specificity: Ubiquitous. Highly expressed in brain. .
• Applications: ELISA,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. Dunning, C. J. R., McKenzie, M., Sugiana, C., Lazarou, M., Silke, J., Connelly, A., Fletcher, J. M., Kirby, D. M., Thorburn, D. R., Ryan, M. T. Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease. EMBO J. 26: 3227-3237, 2007. 2. Fassone, E., Taanman, J.-W., Hargreaves, I. P., Sebire, N. J., Cleary, M. A., Burch, M., Rahman, S. Mutations in the mitochondrial complex I assembly factor NDUFAF1 cause fatal infantile hypertrophic cardiomyopathy. J. Med. Genet. 48: 691-697, 2011. 3. Janssen, R., Smeitink, J., Smeets, R., van den Heuvel, L. CIA30 complex I assembly factor: a candidate for human complex I deficiency? Hum. Genet. 110: 264-270, 2002.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 41409 MW
• Observed Molecular Weight: 36 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Membrane ; Single-pass type I membrane protein .
• Protein Name: Protein NDRG3