Anti-SNF5/SMARCB1 Antibody Picoband®, HRP Conjugate

Anti-SNF5/SMARCB1 Antibody Picoband®, HRP Conjugate

• Background: SMARCB1(SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1), also known as SNF5, INI1 or MALIGNANT RHABDOID TUMOR SUPPRESSOR, is a protein that in humans is encoded by the SMARCB1 gene. The SMARCB1 gene encodes a subunit of the SWI/SNF ATP-dependent chromatin-remodeling complex. The SMARCB1 gene maps to chromosome 22q11.2(Versteege et al., 1998). Wu et al.(2002) noted that GADD34(PPP1R15A) and SNF5 can coexist in a trimeric complex with chimeric leukemic HRX(MLL) fusion proteins, leading to inhibition of GADD34-mediated apoptosis. By mutation analysis, they showed that the GADD34 region homologous to the HSV-1 ICP34.5 protein was necessary for interaction with SNF5. SNF5 could bind independently with the protein phosphatase-1(PP1) catalytic subunit(PPP1CA) and stimulate its activity in solution and in complex with GADD34. SNF5 and PP1 did not compete for GADD34 binding, but rather formed a stable trimeric complex with GADD34. Wu et al.(2002) proposed that GADD34 mediates growth suppression, at least in part, through its interaction with SNF5. They suggested that SNF5 may function as a regulatory subunit of PP1, either independently or together with GADD34.
• Description: Boster Bio Anti-SNF5/SMARCB1 Antibody Picoband® catalog # A00500-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Transcription factor GATA-4;GATA-binding factor 4;GATA4;
• Gene Name: Transcription factor GATA-4
• Gene ID: 6598
• UniProt: Q12824
• Host: Rabbit
• Reactivity: Human,Monkey,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human SNF5/SMARCB1 recombinant protein (Position: E105-D358).
• Clonality: Polyclonal
• Tissue Specificity: Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.
• Applications: WB,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac- specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. van den Munckhof, P., Christiaans, I., Kenter, S. B., Baas, F., Hulsebos, T. J. M. Germline SMARCB1 mutation predisposes to multiple meningiomas and schwannomas with preferential location of cranial meningiomas at the falx cerebri. Neurogenetics 13: 1-7, 2012. 2. Versteege, I., Sevenet, N., Lange, J., Rousseau-Merck, M.-F., Ambros, P., Handgretinger, R., Aurias, A., Delattre, O. Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer. Nature 394: 203-206, 1998. 3. Wu, D. Y., Tkachuck, D. C., Roberson, R. S., Schubach, W. H. The human SNF5/INI1 protein facilitates the function of the growth arrest and DNA damage-inducible protein (GADD34) and modulates GADD34-bound protein phosphatase-1 activity. J. Biol. Chem. 277: 27706-27715, 2002.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 44565 MW
• Observed Molecular Weight: 44 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human, Monkey, Rat
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Nucleus
• Protein Name: Transcription factor GATA-4