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Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband®, HRP Conjugate

CAT:
519-A00175-HRP
Size:
100 µg
Price:
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For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband®, HRP Conjugate - image 1

Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband®, HRP Conjugate

  • Background: Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
  • Description: Boster Bio Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband® catalog # A00175. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Synonyms: Mannose-6-phosphate isomerase;5.3.1.8;Phosphohexomutase;Phosphomannose isomerase;PMI;MPI;PMI1;
  • Gene Name: Mannose-6-phosphate isomerase
  • Gene ID: 4351
  • UniProt: P34949
  • Host: Rabbit
  • Reactivity: Human,Mouse,Rat
  • Cross Reactivity: No cross-reactivity with other proteins.
  • Immunogen: E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.
  • Clonality: Polyclonal
  • Tissue Specificity: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
  • Applications: Flow Cytometry,IHC,WB
  • Purification: Immunogen affinity purified.
  • Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
  • Form: Lyophilized
  • Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
  • Function: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
  • Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
  • References & Citations: 1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998. 2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib). Hum. Mutat. 16: 247-252, 2000.
  • Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Calculated Molecular Weight: 46656 MW
  • Observed Molecular Weight: 47 kDa
  • Fragment: Rabbit IgG
  • Specificity: No cross reactivity with other proteins.
  • Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
    Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
  • Subcellular Location: Cytoplasm .
  • Protein Name: Mannose-6-phosphate isomerase