Anti-TRAP alpha/TRAPA/SSR1 Antibody Picoband®, Dylight550 Conjugate

Anti-TRAP alpha/TRAPA/SSR1 Antibody Picoband®, Dylight550 Conjugate

• Background: Translocon-associated protein subunit alpha is a protein that in humans is encoded by the SSR1 gene. The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.
• Description: Boster Bio Anti-TRAP alpha/TRAPA/SSR1 Antibody Picoband® catalog # A06993-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Aflatoxin B1 aldehyde reductase member 2; AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase; AKR7A2; AFAR; AFAR1; AKR7
• Gene Name: aldo-keto reductase family 7 member A2
• Gene ID: 6745
• UniProt: P43307
• Host: Rabbit
• Reactivity: Human,Monkey,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human SSR1 recombinant protein (Position: E53-E286).
• Clonality: Polyclonal
• Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.
• Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
• References & Citations: 1. Gross, M. B. Personal Communication. Baltimore, Md. 6/22/2012. 2. Hartmann, E., Prehn, S. The N-terminal region of the alpha-subunit of the TRAP complex has a conserved cluster of negative charges. FEBS Lett. 349: 324-326, 1994. 3. Hirama, T., Miller, C. W., Koeffler, H. P. Translocon-associated protein alpha transcripts are induced by granulocyte-macrophage colony-stimulating factor and exhibit complex alternative polyadenylation. FEBS Lett. 455: 223-227, 1999.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 87148 MW
• Observed Molecular Weight: 36 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  
• Subcellular Location: Golgi apparatus. Cytoplasm.
• Protein Name: ADAM metallopeptidase domain 28