Anti-RHOB Antibody Picoband®, Cy3 Conjugate

Anti-RHOB Antibody Picoband®, Cy3 Conjugate

• Background: Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. This gene is mapped to 2p24.1. It is a member of the Rho GTP-binding protein family. And RHOB has been shown to interact with CIT, ARHGEF3, ARHGDIG and RHPN2. RHOB plays a negative role in tumorigenesis as deletion causes tumor formation. Also, it serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis.
• Description: Boster Bio Anti-RHOB Antibody Picoband® catalog # A01550-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Rho-related GTP-binding protein RhoB; Rho cDNA clone 6; h6; RHOB; ARH6; ARHB
• Gene Name: ras homolog family member B
• Gene ID: 388
• UniProt: P62745
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human RHOB recombinant protein (Position: N117-E158).
• Clonality: Polyclonal
• Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
• Applications: WB,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• References & Citations: 1. Cannizzaro, L. A., Madaule, P., Hecht, F., Axel, R., Croce, C. M., Huebner, K. Chromosome localization of human ARH genes, a ras-related gene family. Genomics 6: 197-203, 1990. 2. Chardin, P., Madaule, P., Tavitian, A. Coding sequence of human rho cDNAs clone 6 and clone 9. Nucleic Acid Res. 16: 2717 only, 1988. 3. Madaule, P., Axel, R. A novel ras-related gene family. Cell 41: 31-40, 1985.
• Storage Conditions: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
• Calculated Molecular Weight: 105324 MW
• Observed Molecular Weight: 22 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
  ELISA, 0.1-0.5 μg/ml, -
  
• Subcellular Location: Cell membrane, Lipid-anchor, Late endosome membrane, Nucleus, Cleavage furrow.
• Protein Name: Lysosomal alpha-glucosidase