Anti-NG2/CSPG4 Antibody Picoband®, Cy3 Conjugate

Anti-NG2/CSPG4 Antibody Picoband®, Cy3 Conjugate

• Background: Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
• Description: Boster Bio Anti-NG2/CSPG4 Antibody Picoband® catalog # A03394-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc
• Gene Name: group specific component
• Gene ID: 1464
• UniProt: Q6UVK1
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human NG2/CSPG4 recombinant protein (Position: N962-D1194).
• Clonality: Polyclonal
• Applications: WB,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• References & Citations: 1. Ligon, K. L., Kesari, S., Kitada, M., Sun, T., Arnett, H. A., Alberta, J. A., Anderson, D. J., Stiles, C. D., Rowitch, D. H. Development of NG2 neural progenitor cells requires Olig gene function. Proc. Nat. Acad. Sci. 103: 7853-7858, 2006. 2. Pluschke, G., Vanek, M., Evans, A., Dittmar, T., Schmid, P., Itin, P., Filardo, E. J., Reisfeld, R. A. Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan. Proc. Nat. Acad. Sci. 93: 9710-9715, 1996. 3. Rettig, W. J., Dracopoli, N. C., Goetzger, T. A., Spengler, B. A., Biedler, J. L., Oettgen, H. F., Old, L. J. Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent-human hybrid cells. Proc. Nat. Acad. Sci. 81: 6437-6441, 1984.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 310 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5μg/ml, Human
  ELISA, 0.1-0.5μg/ml, -
  
• Subcellular Location: Secreted.