Anti-RNF43 Antibody Picoband®, Cy3 Conjugate

Anti-RNF43 Antibody Picoband®, Cy3 Conjugate

• Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Description: Boster Bio Anti-RNF43 Antibody Picoband® catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; RNF43
• Gene Name: ring finger protein 43
• Gene ID: 54894
• UniProt: Q68DV7
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).
• Clonality: Polyclonal
• Tissue Specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.
• Applications: ELISA,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• References & Citations: 1. Sugiura, T., Yamaguchi, A., Miyamoto, K. A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95. Exp. Cell Res. 314: 1519-1528, 2008. 2. Yagyu, R., Furukawa, Y., Lin, Y.-M., Shimokawa, T., Yamamura, T., Nakamura, Y. A novel oncoprotein RNF43 functions in an autocrine manner in colorectal cancer. Int. J. Oncol. 25: 1343-1348, 2004.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 86 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot,0.1-0.5μg/ml
  ELISA,0.1-0.5μg/ml
  
• Subcellular Location: Cell membrane; According to a report, may be secreted.