Anti-NMI Antibody Picoband® (monoclonal, 2F3)

Anti-NMI Antibody Picoband® (monoclonal, 2F3)

• Background: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
• Description: Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: N-myc-interactor; Nmi; N-myc and STAT interactor; NMI
• Gene Name: N-myc (and STAT) interactor
• Gene ID: 9111
• UniProt: Q13287
• Host: Mouse
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.
• Clonality: Monoclonal
• Clone: Clone: 2F3
• Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.
• Applications: Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Function: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5.
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• References & Citations: 1.Albihn A, et al. Camptothecin-induced apoptosis is enhanced by Myc and involves PKCdelta signaling. Int J Cancer, 2007 Oct 15. 2.Fillmore RA, et al. Nmi (N-Myc interactor) inhibits Wnt/beta-catenin signaling and retards tumor growth. Int J Cancer, 2009 Aug 1. 3.Lebrun SJ, et al. Interferon-induced upregulation and cytoplasmic localization of Myc-interacting protein Nmi. J Interferon Cytokine Res, 1998 Sep.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 40 kDa
• Fragment: Mouse IgG2b
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
  Immunocytochemistry/Immunofluorescence, 5μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
  
• Subcellular Location: Cytoplasm.